Fig. 4: LRRC19 mediates disruption on intestinal barrier in maternal high-fat diet offspring. | Communications Biology

Fig. 4: LRRC19 mediates disruption on intestinal barrier in maternal high-fat diet offspring.

From: Maternal high-fat diet disrupts intestinal mucus barrier of offspring by regulating gut immune receptor LRRC19

Fig. 4

A Goblet cells in the colon were assessed by PAS staining and the PAS positive cells of each crypt were counted between WT-MHFD and Lrrc19−/−-MHFD groups. The number of positively stained cell in each crypt of five random fields from ×200 images were counted. Scale bar = 50 µm. n = 5 mice per group. B The production of MUC2 in the colon was assessed by immunostaining and the MUC2 positive cells of each crypt were counted in WT-MHFD and Lrrc19−/−-MHFD groups. The number of positively stained cell in each crypt of five random fields from ×200 images were counted. Scale bar = 50 µm. n = 5 mice per group. C The relative expression of MUC2 in mRNA levels was assessed by Realtime-PCR in WT-MHFD and Lrrc19−/−-MHFD groups. n = 3–4 mice per group. D IL-22 levels in the colon were detected by ELISA in WT-MHFD and Lrrc19−/−-MHFD groups. n = 4 per group. E IL-22 levels in the colon were detected by ELISA in in MCD and MHFD groups. n = 5 per group. MCD, maternal control diet. MHFD, maternal high-fat diet. WT, wild type (Lrrc19+/+). All data are displayed as means ± SDs. *p < 0.05, ***p < 0.001, ****p < 0.0001.

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