Fig. 8: IAA ameliorates intestinal barrier function via AhR-ILC3-IL-22-MUC2 axis. | Communications Biology

Fig. 8: IAA ameliorates intestinal barrier function via AhR-ILC3-IL-22-MUC2 axis.

From: Maternal high-fat diet disrupts intestinal mucus barrier of offspring by regulating gut immune receptor LRRC19

Fig. 8

A Experimental flow chart. B, C Colonic lamina propria lymphocytes were collected and the percentages of IL22+RORγT+ ILC3 in colonic lamina propria lymphocytes were analyzed by flow cytometry (B), and the quantification was shown (C). (D) IL-22 levels in the colon were detected by ELISA in WT-Control group, WT-IAA group, Ahr−/−-Control group and Ahr−/−-IAA group. n = 3–5 mice per group. E, G The production of MUC2 in the colon was assessed by immunostaining (G), and the numbers of MUC2 positive cells in each crypt were counted (E). Scale bar = 50μm. n = 5 mice per group. F, H Representative epifluorescence staining for goblet cells in the colon using an antibody against MUC2 (green) and the lectin UEA-I (red) with DAPI (blue) as the counter stain (H). Quantitative analysis of both MUC2 and UEA-I-positive (MUC2+UEA-I+) goblet cell number of per crypt in WT-Control group, WT-IAA group, Ahr−/−-Control group and Ahr−/−-IAA group was counted (F). n = 3–5 mice per group. IAA, indoleacetic acid; MUC2, Mucin-2; WT, wild type (Ahr+/+); ns, not significant. All data are displayed as means ± SDs. *p < 0.05, **p < 0.01, ****p < 0.0001.

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