Fig. 1: Fibrin, a component of haemostatic plugs, is formed outside blood vessels. | Communications Biology

Fig. 1: Fibrin, a component of haemostatic plugs, is formed outside blood vessels.

From: Extravascular coagulation regulates haemostasis independently of activated platelet surfaces in an in vivo mouse model

Fig. 1

Representative images of haemostatic plug formation after venous (a) and arterial (b) internal bleeding. The mice were intravenously injected with dextran-rhodamine B, Alexa Fluor 488-conjugated fibrinogen and the platelet imaging antibody X649 prior to observation. After two-photon excitation injury (circle of dots), platelet aggregate formation in the lumen (magenta triangle) and extravascular plasma leakage (red triangle) were observed. After arterial internal bleeding, red blood cell leakage was observed (white asterisk). Fibrin is formed in the extravascular area (green triangle). The haemostatic plug consisted of a platelet aggregate in the lumen, extravascular fibrin, and platelets and fibrin at the boundary of the lumen and extravascular space (white triangle). Scale bar = 20 μm. Green: fibrin/fibrinogen. Magenta: platelets. Red: plasma.

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