Fig. 4: Irreversible platelet activation does not occur in early haemostatic plugs. | Communications Biology

Fig. 4: Irreversible platelet activation does not occur in early haemostatic plugs.

From: Extravascular coagulation regulates haemostasis independently of activated platelet surfaces in an in vivo mouse model

Fig. 4

Representative images of platelets that clog the liver vasculature after systemic thrombin injection (a) and haemostatic plugs that formed after large arterial injury (b). For arterial haemostatic plug observation, the mice were injected with Alexa Fluor 488-conjugated fibrinogen, PE-conjugated rat anti-mouse CD62P antibody, and platelet imaging antibody X649 prior to observation. For platelet observation after thrombin injection, the mice were injected with FITC-dextran, PE-conjugated rat anti-mouse CD62P antibody, and X649 antibody prior to observation. CD62P expression was detected on platelets that clog the liver vasculature (a) but was not detected in the in vivo internal bleeding/haemostasis model (b). Scale bar = 20 μm. Green: fibrin/fibrinogen. Magenta: platelets. Red: plasma.

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