Fig. 7: Extravascular coagulation is important for platelet aggregate formation and the cessation of plasma leakage after venous internal bleeding.

Measurements of platelet aggregates, fibrin, and plasma leakage were taken from experimental mice (a) (wild-type, n = 6 lesions from 5 animals; Ano6 cKO, n = 5 lesions from 5 animals; platelet-depleted, n = 5 lesions from 5 animals; FVIII KO, n = 6 lesions from 4 animals; and L-TF mice, n = 16 lesions from 4 animals). Platelet aggregates were assessed by height from the vascular endothelium. Fibrin was quantified as the ratio of the fibrin area over time, normalized to the end of the observation period. Plasma leakage was expressed relative to the maximum signal outside the vessel. Box-and-whisker plot of the peak platelet aggregate height (b). Peak platelet aggregate height at 0–120 and 120–300 s (c). The increase in the fibrin area accelerated, as indicated by the ratio (d). Time required for the fibrin area to reach half the area at the end of observation (e). Area under the curve (AUC) of plasma leakage (f). *<0.05, **<0.01. c Wilcoxon signed-rank test; b, d–f Steel–Dwass test and Monte Carlo simulation.