Fig. 8: Extravascular coagulation is important for the formation of platelet aggregates and the cessation of blood cell and plasma leakage after arterial internal bleeding.

Measurements of platelet aggregates, fibrin, and plasma leakage were taken from experimental mice (a) (wild-type, n = 6 lesions of 6 animals; Ano6 cKO, n = 7 lesions of 6 animals; platelet-depleted, n = 5 lesions of 5 animals; FVIII KO, n = 5 lesions of 5 animals; and L-TF mice, n = 6 lesions of 6 animals). Platelet aggregates were assessed by height from the vascular endothelium. Fibrin was quantified as the ratio of the fibrin area over time, normalized to the end of the observation period. Plasma leakage was expressed relative to the maximum signal outside the vessel. Box-and-whisker plot of the peak platelet aggregate height (b). Peak platelet aggregate height at 0–120 and 120–300 s (c). The increase in the fibrin area accelerated, as indicated by the ratio (d). Time required for the fibrin area to reach half the area at the end of observation (e). Area under the curve (AUC) of plasma leakage (f). The time from the start of bleeding to the cessation of blood cell leakage (g). The extent of platelet aggregate extrusion from the vessel was evaluated by measuring the distance from the vessel wall to the platelets, as shown in (h). †<0.1, *<0.05, **<0.01. c Wilcoxon signed-rank test; b, d–h Steel–Dwass test and Monte Carlo simulation.