Fig. 4: RBPMS and ANKRD10 affect BLCA progression by regulating MYC target gene expression. | Communications Biology

Fig. 4: RBPMS and ANKRD10 affect BLCA progression by regulating MYC target gene expression.

From: RBPMS inhibits bladder cancer metastasis by downregulating MYC pathway through alternative splicing of ANKRD10

Fig. 4

a RNA-seq data were analyzed using GSEA enrichment analysis, and pathways upregulated by RBPMS knockout and ANKRD10-2 overexpression were intersected with pathways downregulated by ANKRD10-2 knockdown. The pathways obtained by GSEA enrichment were selected as 10 with the smallest p.adjust values. b Hallmark_MYC_Targets_V1 gene set demonstrated by GSEA enrichment of all RNA-seq results. c Heatmap of Hallmark_MYC_Targets_V1 genes in RNA-seq results of knockout RBPMS. d 5637 sgCtrl and sgRBPMS cells were transfected with the E-box dual-luciferase plasmid and then assayed for reporter fluorescence activity (n = 3). e Validation of differential mRNA levels of MYC target genes in RNA-seq assays after RBPMS knockout by qRT-PCR (n = 3). f After overexpression of RBPMS-A or RBPMS-C, 5637 sgCtrl and sgRBPMS cells were transfected with the E-box dual-luciferase plasmid and assayed for reporter fluorescence activity (n = 3). g mRNA levels of MYC target genes in 5637 sgCtrl and sgRBPMS cells detected by qRT-PCR after overexpression of RBPMS-A or RBPMS-C (n = 3). Data are shown as mean ± SD. The n number represents n biologically independent experiments in each group.

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