Fig. 5: ANKRD10-2 interacts with MYC to promote its transcriptional regulatory function on target genes. | Communications Biology

Fig. 5: ANKRD10-2 interacts with MYC to promote its transcriptional regulatory function on target genes.

From: RBPMS inhibits bladder cancer metastasis by downregulating MYC pathway through alternative splicing of ANKRD10

Fig. 5

a GSEA enrichment of RNA-seq data after ANKRD10 knockdown in 5637 sgRBPMS cells demonstrates a positive correlation with ANKRD10-2 in the transcriptional coactivator and coregulator activity pathways. b ANKRD10-2 knockdown in 5637 sgRBPMS cells was subjected to RNA-seq and enriched by GSEA using the GO database. c After ANKRD10-2 knockdown, 5637 sgRBPMS cells were transfected with an E-box dual-luciferase plasmid and assayed for reporter fluorescence activity (n = 3). d mRNA levels of MYC target genes in 5637 sgRBPMS cells were detected by qRT-PCR after ANKRD10-2 knockdown (n = 3). e After ANKRD10-2 overexpression, 5637 sgRBPMS cells were transfected with an E-box dual-luciferase plasmid and assayed for reporter fluorescence activity (n = 3). f mRNA levels of MYC target genes in 5637 cells were detected by qRT-PCR after ANKRD10-2 overexpression (n = 3). Transfection of 293 T cells with the specified plasmids lasted 48 h, followed by Co-IP using either anti-FLAG (g) or anti-HA antibodies (h). i ChIP-qPCR analysis examined MYC binding to promoters of C1QBP, DDX21, HK2, PGK1, PRMT3, RSL1D1 and WDR43 in 5637 cells after ANKRD10-1 or ANKRD10-2 knockdown (n = 3). Data are shown as mean ± SD. The n number represents n biologically independent experiments in each group.

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