Fig. 3: The effect of GBP5 knockout on the global mRNA expression mirrors that of STAT1 activation in THP-1 cells.

a Plots are the mRNA expression data of STAT1 and STAT1 target genes from RNA sequencing-based transcriptome analysis. Wildtype and GBP5^-/- THP-1 cells were treated with IFNγ (25 ng/ml) plus LPS (500 ng/ml) or mock-treated for 16 h. The list of the STAT1 target genes was from TRRUST version 2, with a few others (CCND1, MYC, and CDC25A) added according to the literature. Genes known to be stimulated and repressed by STAT1 are indicated by red and green, respectively. n = 3 biologically independent samples for each group. b Gene set enrichment analysis (GSEA) of the entire transcriptome data of GBP5^-/- cells compared to wild-type THP-1 cells. The upper panel is the enrichment score curve for JAK-STAT signaling pathway, showing the decreased expression level of relevant genes in Gbp5 deficient cells. The middle panel shows the distribution of the genes related to JAK-STAT signaling pathway. The genes were ranked according to their differential expression between wild-type and GBP5^-/- cells. The lower panel is a graphical representation of the correlations between gene expression levels and the genotypes: WT or GBP5^-/-. NES, normalized enrichment scores; FDR, false discovery rate.