Fig. 3: BG-11 medium component concentration and antibiotic sensitivity testing in Synechococcus elongatus UTEX 2973. | Communications Biology

Fig. 3: BG-11 medium component concentration and antibiotic sensitivity testing in Synechococcus elongatus UTEX 2973.

From: An automated high-throughput lighting system for screening photosynthetic microorganisms in plate-based formats

Fig. 3

To demonstrate applicability of the lighting system, the impact of individually varying the concentration of each of the 8 components of BG-11 medium was tested. 8 components at 11 concentrations and with 7 replicates per concentration were distributed randomly across sections 2–8 of two black-well microplates, resulting in 716 individual cultures. A Growth curves show impact of nutrient limitation over the course of a 120 h experiment. B Response curves at T3 (72 h) show limiting nutrients and tight condition-dependent clustering of OD750 values. Sensitivity to 4 antibiotics was tested at 11 concentrations and with 7 replicates per concentration. C Growth curves show impact of on growth rate over the course of a 120 h experiment. D Response curves at T3 (72 h) show antibiotic sensitivity and tight condition-dependant clustering of OD750 values.

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