Fig. 4: BG-11 medium optimization and response surface modelling. | Communications Biology

Fig. 4: BG-11 medium optimization and response surface modelling.

From: An automated high-throughput lighting system for screening photosynthetic microorganisms in plate-based formats

Fig. 4

BG-11 optimization and RSM were performed through a 61-condition, 8-factor central composite design experiment. A Response surface model showing the relationships between components NaNO3 and MgSO4 and components CaCl2 and K2HPO4, as well as their predicted impact of growth. B Predicted effects of individual medium components on growth of each strain with or without NaHCO3. Values are plotted as LogWorth = −log10(p-value). C Flask-based cultivation in standard and optimized BG-11 media showed reproducible increases in biomass accumulation over 7-day cultivation experiments. Points represent biomass from individual 50 mL flask cultures. Green bars represent mean biomass of three flasks in standard and optimized medium.

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