Fig. 5: High-throughput bioactive molecule screening and gradient testing of select candidate compounds.
A Round 1 of bioactive molecule screen was run in two sets, monitoring OD750 values in each well. Shades of grey indicate OD750 from 14 384-well plates. Solid lines represent median OD750 values for each set. B Plate-by-plate Z-scores were calculated to normalize data and identify outliers. Colour represents selected outliers (dark green). Solid line represents Z-score of 0. Dashed lines represent Z-score of ± 2.5. Data showed near normal distribution. C All OD750 data points from round 2 screening. 146 hits (each in triplicate) and 396 negative control wells, across three 384-well plates. D Selected hits from round 2 with negative control wells. Negative control data showed a positively skewed distribution. E Round 3 gradient testing for selected hits inducing growth and F inhibiting growth. Line colours indicate selected compounds for downstream testing. G Lines show a median of 8–12 replicate wells at each concentration of selected candidate compounds. H Fold-change in OD750 compared to negative control wells (no gossypetin treatment), showing 12 replicates at each concentration [non-linear x-axis]. I Median (solid), 25th percentile (dashed), and 75th percentile (dashed) OD750 measurements across 15 gossypetin concentrations [linear x-axis]. J Flask-based cultivation at 10 μM and 20 μM gossypetin showed reproducible increases in biomass accumulation over a 7-day cultivation experiment.
