Fig. 1: SunTag-PE system enables efficient prime editing in HEK293T cells.
From: SunTag-PE: a modular prime editing system enables versatile and efficient genome editing
a SunTag-PE consists of GCN4 fused to the N-terminus of nCas9. b PE-SunTag consists of GCN4 fused to the C-terminus of nCas9. c Two-plasmid system of SunTag-PE, one plasmid expressing pegRNA and scFv-RT and the other expressing n×GCN4 tethered nCas9. d Different configurations of SunTag-PE and PE-SunTag (the copy number of GCN4 was 1, 2, 3, 5, and 10). e Comparison of CTT insertion efficiencies of different SunTag-PE2 and PE2-SunTag configurations in HEK3 locus by deep sequencing. f Comparison of CTT insertion efficiencies of SunTag-PE2 with N-1×GCN4, deficient SunTag-PE2, Split-PE2, MS2-PE2, and canonical fused PE2 in HEK3 locus. Data and error bars indicate the mean and s.d. of three independent biological replicates. Negative controls represent groups only with pegRNAs, untreated with PE components. The numbers above the bars represent fold change between different treatment groups.
