Fig. 5: Recovery of diabetic corneal nerve regeneration with chemical sympathectomy. | Communications Biology

Fig. 5: Recovery of diabetic corneal nerve regeneration with chemical sympathectomy.

From: Norepinephrine regulates epithelial-derived neurotrophins expression and sensory nerve regeneration through ADRB2 receptor

Fig. 5

DM mice were intraperitoneally injected with 6-OHDA and subsequently debrided the central corneal epithelium. The corneas of healthy, DM, and 6-OHDA-treated DM mice were collected at 48 h after epithelial debridement. A NGF and GDNF mRNA transcripts were monitored with qPCR (n = 3 mice per group). B NGF and GDNF protein levels were measured with an ELISA (8 corneas pooled per group). C Immunofluorescence staining of NGF and GDNF in the cornea. D The fluorescence intensity of NGF and GDNF in the corneal epithelium was quantified by Image J software. E Mouse corneal nerves were stained with the Alexa Fluor® 488-labeled α-βIII-tubulin antibody at 48 h post wounding. F The subbasal nerve fibers were counted with Image J software (n = 4 corneas per group). G Corneal sensations were measured with a Cochet-Bonnet esthesiometer (n = 4 mice per group). Data are representative of the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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