Fig. 6: Mediation of ADRB2 on the regulation of NE on NGF and GDNF expressions. | Communications Biology

Fig. 6: Mediation of ADRB2 on the regulation of NE on NGF and GDNF expressions.

From: Norepinephrine regulates epithelial-derived neurotrophins expression and sensory nerve regeneration through ADRB2 receptor

Fig. 6

A Relative expression of ADRs in the mouse cornea. B–E NGF and GDNF mRNA transcripts and protein levels were detected with qPCR and ELISA in HCECs treated with PBS, NE, NE and atenolol (ATE), and NE and ICI 118, 551 (ICI). F Scheme of indirect cell culture of TKE2 cells and TG neurons. G Representative TG neurite outgrowth incubated with the various conditioned media of TKE2 cells treated with PBS, NE, NE and ATE, and NE and ICI. The cultured TG neurons were stained with Alexa Fluor® 488-labeled βIII-tubulin antibody. H The TG neurite outgrowth was analyzed with Neuro J software. Data are representative of the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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