Fig. 7: The ADRB2 antagonist accelerates corneal wound healing in DM mice. | Communications Biology

Fig. 7: The ADRB2 antagonist accelerates corneal wound healing in DM mice.

From: Norepinephrine regulates epithelial-derived neurotrophins expression and sensory nerve regeneration through ADRB2 receptor

Fig. 7

DM mice were performed with corneal epithelial debridement and topically applied with the eye drop containing 10 μM ICI 118, 551 (ICI). A, B Mouse corneal epithelial defects were monitored with fluorescein staining and calculated with Image J software (n = 5 mice per group). C, D Mouse corneal nerves were stained and the subbasal nerve fibers were counted with Image J software at 48 h post wounding (n = 4 corneas per group). E Corneal sensations were measured with a Cochet-Bonnet esthesiometer at 48 h post wounding (n = 5 mice per group). Corneal NGF and GDNF expressions were detected with qPCR (n = 3 mice per group) at 48 h post wounding (F) and immunofluorescence staining (G). H The fluorescence intensity of NGF and GDNF in the corneal epithelium was quantified by Image J software. Data are representative of the mean ± SD. *p < 0.05, **p < 0.01, ***P < 0.001, ****P < 0.0001.

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