Fig. 2: POT-PI3K induces endogenous protein degradation through the TRIM21-mediated proteasomal pathway.
From: POT, an optogenetics-based endogenous protein degradation system
a A549 cells expressing pLenti-EF1a (empty vector), POTΔTRIM or POT-PI3K were illuminated with or without blue light LED array (1 s every 6 s, 1 mW/cm2) for 4 h before immunoblotting. All groups were pretreated with cycloheximide (CHX; 10 μg/ml) for 2 h before experiments. b–f The normalized densitometric of proteins over GAPDH ratio and pAkt473 over Akt ratio (e) in five independent experiments. g COS-7 cells expressing POT-PI3K were illuminated with or without blue LED array (1 s every 6 s, 1 mW/cm2) and treated with (R)-MG132 for 4 h before immunoblotting. h The normalized densitometric PI3K 110α (left) and POT-PI3K (right) over GAPDH ratio in three independent experiments. All data were presented as mean ± SEM. Statistical significance is based on One-way ANOVA tests and is represented with labels, ns (P > 0.05), *(P ≤ 0.05), **(P ≤ 0.01), ****(P ≤ 0.0001).
