Fig. 4: Degradation of endogenous PI3K 110α by POT-PI3K inhibits cell migration, proliferation and promotes cell apoptosis.
From: POT, an optogenetics-based endogenous protein degradation system
a Representative images of HeLa cell migration were assessed by wound healing assay. Cells expressing pLenti-EF1a, POT-PI3K, or POTΔTRIM were illuminated with or without blue light LED array (1 s every 6 s). HeLa cells treated with LY294002 and insulin were used as experimental controls. The images were captured at 48 h after wound scratch. Scale bar, 500 μm. b Quantified results of the wound healing assay are presented (n = 3 independent samples). c CCK-8 assay was employed to quantify the HeLa cell proliferation under different treatments (n = 3 independent samples). d HeLa cell apoptosis was analyzed by flow cytometry under different treatments and quantitatively plotted in (e) (n = 3 independent samples). All data were presented as mean ± SEM. Statistical significance is based on One-way ANOVA tests and is represented with labels, * (P ≤ 0.05), ** (P ≤ 0.01), ***(P ≤ 0.001), **** (P ≤ 0.0001).
