Fig. 1: Genetic deletion of TLR4 exhibits wide transcriptomic alterations in the lungs following sepsis.

A UMAP visualization of a total of 18,152 cells from all 14 lungs sample (WT Sham n = 3, Tlr4-/- Sham n = 4, WT CLP 24 h n = 4, and Tlr4-/- CLP 24 h n = 3), clustered into 7 cell types based on relative markers. B The number of upregulated and downregulated DEGs across specific cell types in the WT CLP group compared to the Tlr4-/- CLP group. C The gene expression changes between the Tlr4-/- CLP and WT CLP groups. The x-axis represents the difference in expression proportion and the y-axis shows the log2 fold change. D The pathways that are upregulated in the WT CLP group compared to the Tlr4-/- CLP group. The x-axis represents the negative logarithm of the p-value (-Log10P), which indicates the statistical significance of the upregulation. Each bar shows the extent of pathway enrichment, with longer bars indicating stronger significance. Specific genes involved in each pathway are listed next to the pathway name.