Fig. 4: Identification of PTOV1 Binding Proteins in CRC Cells. | Communications Biology

Fig. 4: Identification of PTOV1 Binding Proteins in CRC Cells.

From: PTOV1 interacts with ZNF449 to promote colorectal cancer development

Fig. 4

A Protein analysis report to characterize PTOV1 binding partners. B Identification of PTOV1-binding proteins by WB and MS. C, D Using Co-IP and GST pull-down to clarify that PTOV1 can directly bind ZNF449. E IF staining revealed coexpression of PTOV1 and ZNF449 in CRC cells. Bars: 20 μm. F IF staining showed the colocalization (yellow) between ZNF449 (green) and PTOV1 (red) in CRC sections. Bars: 50 μm.

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