Fig. 5: PTOV1 promotes ZNF449-mediated transcription of MYC.
From: PTOV1 interacts with ZNF449 to promote colorectal cancer development
A We constructed the wild-type or mutant-type luciferase vectors based on the potential binding site of ZNF449 to the MYC promoter. B Luciferase activity was assayed in SW48 and SW620 cells transfected with luciferase vectors (wild type or mutant type) and meantime co-transfected with expression plasmids (empty vectors, ZNF449 expression plasmids, or PTOV1 expression plasmids). C ChIP experiments of ZNF449 (IgG as an internal control) were performed, and the co-precipitated DNA was subjected to PCR amplification with primers specific to MYC promoter region. D The level of MYC under ectopic expression of ZNF449 or PTOV1 was detected by qRT-PCR and WB. The means ± SDs are provided (n = 3). **P < 0.01 and ***P < 0.001 according to two-tailed Student t-tests or one-way ANOVA followed by Dunnett tests for multiple comparisons. ns, no significant difference.
