Fig. 1: Reconstructing endogenous trichromatic-fluorescence-labeled chimeric mice.

A Schematic representation of the Krt14-YFP/Lyz2-RFP/CX3CR1-GFP BM chimeric mouse reconstitution and the OXA-induced ICD procedure. B Representative confocal images of OXA-challenged chimeric mouse skin in different depths. GFP (monocytes), RFP (neutrophils), EYFP (keratinocytes), scale bar: 50  μm. C, D representative flow cytometry images of skin-infiltrated myeloid cells. C The proportions of monocytes/neutrophils in GFP⁺ or RFP⁺ cells. D Proportions of GFP and RFP expression in monocytes and neutrophils. E Quantitative of neutrophils proportions in RFP⁺GFP^- cells. F Monocytes proportions in GFP⁺ cells. Quantitative data of RFP⁺ or GFP⁺ proportions in neutrophils (G) and monocytes (H). n = 9 in (E, F), n = 12 in (G, H). All the mice were female for these experiments. Data are presented as mean ± SD from three independent experiments.