Fig. 3: Monocytes mediate the NTEM in the skin. | Communications Biology

Fig. 3: Monocytes mediate the NTEM in the skin.

From: The crosstalk of monocyte-neutrophil in hair follicles regulates neutrophil transepidermal migration in contact dermatitis

Fig. 3

A Representative flow cytometry image displaying neutrophils and monocytes infiltration in epidermis and dermis in PBS-Lipo or Clod-Lipo-treated group. B Ear thickness in PBS-Lipo or Clod-Lipo-treated group (n = 9, means ± SEM). C Quantitative data of monocyte and macrophage counts in the dermis of PBS-Lipo or Clod-lipo-treated group (n = 12, means ± SEM). D Two-photo imaging of OXA-irritated chimeric reporter mouse skin in Clod-Lipo-treated (upper panel) or PBS-Lipo (lower panel) group. Image showing neutrophils and monocytes infiltration at 30, 40, 50, and 60 μm below the epidermis. Green (monocyte/GFP), Red (neutrophil/RFP), and Blue (keratinocyte/YFP). E Quantitative data of neutrophils counts in epidermis and dermis (n = 12, means ± SEM). F Flow cytometry analysis of epidermal/dermal infiltrated neutrophil ratio (NTEM ratio), n = 9, means ± SEM. All the mice were female for these experiments. Data are representative of three or more independent trials. Statistical analysis was performed using a two-tailed unpaired Student’s t-test.

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