Fig. 5: TNF-α and CXCL2 induce MMP-9 expression in neutrophils.

A ELISA detection of skin TNF-α production in PBS-Lipo or Clod-Lipo-treated group. n = 3, means ± SEM. B ELISA detection of skin CXCL2 production in PBS-Lipo or Clod-Lipo-treated group. n = 3, means ± SEM. C qPCR examination of Tnf expression in skin infiltrated cells. n = 9, means ± SEM. D qPCR examination of Cxcl2 expression in skin infiltrated cells. n = 9, means ± SEM. E Flow cytometry analysis of NTEM ratio in Tnf-deficient and Tnf-competent mice. n = 9, means ± SEM, 3 mice were excluded due to the technical complications. F qPCR analysis of Cxcl2 expression in Tnf-deficient and Tnf-competent mice. G qPCR analysis of Mmp9 expression Tnf-deficient and Tnf-competent mice. n = 9, means ± SEM. H Epidermis/dermis NTEM ratio in CXCR2 inhibitor or vehicle-treated group. n = 9, means ± SEM. I Epidermal/dermal infiltrated neutrophil ratio (NTEM ratio) in anti-CXCL2 or IgG treated group. n = 12, means ± SEM. J, qPCR analysis of Mmp9 expression in anti-CXCL2 or IgG treated group, n = 6–9. Data are representative of two independent experiments in (C, D, J). All the mice were female for these experiments. Data are representative of three independent experiments in (E–I). Statistical analysis was performed using a two-tailed unpaired Student’s t-test in (A, B, E–J). Data in (C, D) were analyzed using One-way ANOVA followed by Bonferroni’s post hoc test. Bonferroni-corrected p values are indicated in (C, D).