Fig. 1: snRNA-seq profiles of BWS and nonBWS livers. | Communications Biology

Fig. 1: snRNA-seq profiles of BWS and nonBWS livers.

From: Single-nucleus multiomic analysis of Beckwith-Wiedemann syndrome liver reveals PPARA signaling enrichment and metabolic dysfunction

Fig. 1

a Schematic of our multiomics study. Single nuclei were isolated from 4 BWS and 3 nonBWS tumor-adjacent liver samples and prepared for snRNA-seq using the 10X Genomics technology. Created with BioRender.com b UMAP visualization 74,315 liver nuclei isolated from 7 liver samples. c The uniform manifold approximation and projection (UMAP) of BWS (n = 4) and nonBWS (n = 3) livers using snRNA-seq demonstrates that both liver groups contain the same cell types clustered into 18 cell subpopulations. This transcriptome atlas identified major liver cell types including hepatocytes, cholangiocytes, hepatic stellate cells, Kupffer cells, endothelial cells and immune system cells in both BWS and nonBWS livers. Dot plots of snRNA-seq dataset showing gene expression patterns of cluster-enriched markers for BWS liver (d) and nonBWS livers. e The diameter of the dot corresponds to the proportion of cells expressing the indicated gene and the density of the plot corresponds to average expression relative to all cell types. f Stacked column graphs displaying the average cell type distribution percentages between BWS (left) and nonBWS (right) livers. The percentages from each individual color add up to the total number of cells in that sample type.

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