Fig. 5: Hepatocyte-specific BWS effects on PPARA signaling and fatty acids in vitro. | Communications Biology

Fig. 5: Hepatocyte-specific BWS effects on PPARA signaling and fatty acids in vitro.

From: Single-nucleus multiomic analysis of Beckwith-Wiedemann syndrome liver reveals PPARA signaling enrichment and metabolic dysfunction

Fig. 5

GO term enrichment plot for Day 15 of hepatocyte differentiation in BWS 2 (a) and BWS 1 (b) line when compared to Day 15 of hepatocyte differentiation in the control line. GO term enrichment plot for Day 20 of hepatocyte differentiation in BWS 2 (c) and BWS 1 (d) line when compared to Day 20 of hepatocyte differentiation in the parental line. Live cell imaging of control, BWS 1, and BWS 2 cells undergoing differentiation and stained with Bodipy (lipid droplets) (e), FAO Blue (fatty acid β-oxidation) (f), and Acrolein Red (lipid peroxidation) (g). Quantification for days 15, 18, and 20 of differentiation are shown beside the image and represent the mean ± SEM of the background-corrected raw intensity value divided by cell number. p values were generated by one way ANOVA. At least 2 biological replicates are displayed per time point.

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