Fig. 2: Species-specific expression of genes encoding nAChRs in the pituitary of Siberian tiger.
From: Nicotinic acetylcholine receptors regulate growth hormone in pituitary somatotrophs of tigers
a t-SNE plot showing somatotrophs atlas of Siberian tiger and mouse pituitary gland. Insert shows expression of CHRNA3, CHRNB4, and CHRNA5 encoding nAChRs. b Comparison of CHRNA3, CHRNB4, and CHRNA5 differential expression in somatotrophs between the Siberian tiger and the mouse. c Expression level of CHRNA3, CHRNB4, and CHRNA5 genes in the pituitary gland is shown in the left panel for adult Siberian tiger (n = 1), domestic cat (n = 3), mouse (n = 6), and rat (n = 6), and in the right panel for juvenile domestic cat (n = 3), mouse (n = 6), and rat (n = 6). d Expression level of CHRNA5 protein in the pituitary gland of Siberian tiger (n = 1), domestic cat (n = 3), mouse (n = 6) and, rat (n = 6), assessed by Western blot on non-denaturing gels. e Representative immunofluorescence of GH and CHRNA5 (α5) in the pituitary of a Siberian tiger cub (top) and a mouse (bottom). The panels display confocal images of GH (green), α5 (red), DAPI-stained nuclei (blue), and merged images showing dual fluorescence labeling with DAPI. Scale bar: 10 μm.
