Fig. 3: Activation of nAChRs in the Siberian tiger.
From: Nicotinic acetylcholine receptors regulate growth hormone in pituitary somatotrophs of tigers
a, b Whole cell currents of Siberian tiger α3β4 and α3β4α5 activated by 100 μM acetylcholine. All patches were recorded at a holding potential of −70 mV. c Concentration-response relationships of Siberian tiger α3β4 and α3β4α5 for acetylcholine fitted to a Hill equation (n = 5 per data point). All patches were recorded at a holding potential of −70 mV. d, e Current-voltage relationship of various cations for α3β4 and α3β4α5-transfected cells. f Reversal potentials of different cations for α3β4 and α3β4α5-transfected cells (n = 5 cells for each group). α3β4 Erev (Na+ k+ Ca2+) = 6.4 ± 0.6 mV, 6.1 ± 0.9 mV, 5.4 ± 0.8 mV, respectively; α3β4α5 Erev (Na+ k+ Ca2+) = 6.6 ± 1.6 mV, 5.1 ± 1.2 mV, 9.8 ± 2.2 mV, respectively. g Ion permeabilities for α3β4 and α3β4α5 activated by acetylcholine (n = 5 cells per group). α3β4 (PNa/PCs = 1.33; Pk/PCs = 1.30; PCa/PCs = 0.90); α3β4α5 (PNa/PCs = 1.20; Pk/PCs = 1.23; PCa/PCs = 0.86). All statistical data are given as average ± SEM.
