Fig. 2: Ab-dependent NR2F1 localization was tested in different cell types. | Communications Biology

Fig. 2: Ab-dependent NR2F1 localization was tested in different cell types.

From: Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivo

Fig. 2

Gray boxes show the selected zoom-in areas. A IF of NR2F1 by Ab H8132 (green) or by Ab #6364 (red) was performed in HeLa cells, hiPSC-derived hNCC, and undifferentiated hiPSC. Nucleolar-like foci stained by Ab H8132 are indicated by arrowheads. Ab #6364 stains endogenous NR2F1 only in the nucleoplasm and specifically in HeLa and hNCC, but not in hiPSC, which do not express NR2F1. DAPI marks necleoplasm (blue). B IF of NR2F1 Ab H8132 (green), Hoechst (blue), and two different nucleolar markers, Fibrillarin (red) and NPM1 (red). C Live-cell imaging of hiPSCNR2F1-GFP 24 h after transfection shows nuclear but not nucleolar NR2F1 localization. Zoom-in is marked with a white box and shown for the GFP channel below. Arrowheads point to NR2F1-GFP-negative nucleoli. Scale bars: 50 µm.

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