Fig. 9: Fibrinogen and gelatin are substrates for the peptidase SpBcA (30–601 aa).
From: Functional study of Bergeyella cardium KP-43 subfamily peptidases as putative T9SS cargo
Degradation of fibrinogen by A and A-H160A over time. Fibrinogen (final concentration of 1.91 µg/µL) was preincubated at a final concentration of 0.191 µg/µL peptidase A (a) or A-H160A (b) in a 115 μL reaction mixture containing 100 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2 and ddH2O, pH 7.4. At the indicated time points, aliquots (10 μL) were taken from the reaction mixture and processed as described in “Methods” section. c Fibrinogen (final concentration of 1.91 µg/µL) was used as a negative control in 115 μL of buffer, which consisted of 100 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2 and ddH2O, pH 7.4. d Degradation of gelatin by A. Gelatin was preincubated with peptidase A and the positive control (+) (collagenase IV) provided with the kit. e Degradation of hemoglobin by A. Hemoglobin (final concentration of 0.96 µg/µL) was preincubated with peptidase A (final concentration of 0.096 µg/µL) in 115 μL of 100 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2 and ddH2O, pH 7.4. At the indicated time points, aliquots (10 μL) were taken from the reaction mixture and processed as described in “Methods” section.
