Fig. 1: Extended associativity in strong-before-weak synaptic tagging and capture paradigm.
From: Beyond boundaries: extended temporal flexibility in synaptic tagging and capture
a Schematic representation of a transverse hippocampal slice showing the location of electrodes in the CA1 region. Recording electrode (rec) positioned onto CA1 apical dendrites was flanked by two stimulating electrodes, S1 and S2, placed in the stratum radiatum (sr) layer to stimulate two independent Schaffer collateral (sc) synaptic inputs of the same neuronal population. b A weak tetanization (WTET, single train of 21 pulses at 100 Hz) applied to S2 (blue circle) induced a significant potentiation that gradually decayed to baseline level (n = 7 slices from 5 animals). The control input S1 (red circles) remained stable throughout the recording period. Strong-before-weak STC paradigm where a strong tetanization (STET, 3 trains of 100 pulses at 100 Hz, inter-trains interval of 10 min) was applied to S1 (red circles) followed by a WTET applied to S2 (blue circles) at various intervals, (c) 0.5 h (n = 8 slices from 5 animals), (d) 3 h (n = 7 slices from 6 animals), (e) 6 h (n = 7 slices from 7 animals), (f) 9 h (n = 7 slices from 5 animals). The LTPs induced in S1 and S2 maintained at potentiated level throughout the recording period in all cases. Analogue traces show typical fEPSP of S1 (red) and S2 (blue) at 15 min of baseline (dotted line), 10 min after LTP induction (dashed line), and at the end of the recording (solid line). Scale bar for all traces: 3 mV/5 ms. Three red arrows represent STET, whereas single blue arrow represents WTET. All data are represented as mean ± SEM.
