Fig. 4: Schematic workflow for differentiating between 5mC and 5hmC modifications using M.SssI-treated and Ligation-based DNA. | Communications Biology

Fig. 4: Schematic workflow for differentiating between 5mC and 5hmC modifications using M.SssI-treated and Ligation-based DNA.

From: Transformer-based deep learning for accurate detection of multiple base modifications using single molecule real-time sequencing

Fig. 4

A Illustration of the composition of TET-treated DNA. B Illustration of the preparation for the 5hmC detection dataset (named Lig-5hmC) based on a ligation method. C The analytical workflow for 5mC and 5hmC detection in SMRT-seq. D ROC curves of the testing datasets for the 5xC and 5hmC detection. E Box plots of modification scores for 5hmC detection in the testing dataset.

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