Fig. 7: SERCA2a undergoes autophagy degradation during TMAO-induced cardiac hypertrophy in mice. | Communications Biology

Fig. 7: SERCA2a undergoes autophagy degradation during TMAO-induced cardiac hypertrophy in mice.

From: The gut microbiota metabolite trimethylamine N-oxide promotes cardiac hypertrophy by activating the autophagic degradation of SERCA2a

Fig. 7

C57BL/6 J were fed with TMAO or vehicle, and then injected intraperitoneally with 3MA for 2 weeks before being sacrificed. a Representative M-mode echocardiography of the left ventricle. Measurement of EF% and FS% (b, c), n = 4. d–f Representative images of heart size photographed with a stereomicroscope and HW/BW and HW/TL ratios, n = 8. g Cardiac tissue size and fibrosis were detected by FITC-labeled WGA staining (60×), HE staining (40×), and Masson staining (20×). h Representative images of autophagosomes detected by transmission electron microscopy in the heart tissues. Red arrow: autolysosome; yellow arrow: autophagosome. i–p Western blot analysis (n = 6) of LC3II, p62, ATG5, SERCA2a, MYH7, and ANP in the left ventricle tissues. Statistical analyse was performed using the One-way ANOVA with Tukey’s multiple comparisons test. Error bars represent S.E.M.

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