Fig. 1: Leaf interveinal segmentation and microscopic visualization of chloroplast clusters and stomata.
From: Spatial patterning of chloroplasts and stomata in developing cacao leaves
a Interveinal segmentation from top to bottom on each left (L) or the right (R) half of the abaxial side of a representative cacao leaf (genotype Pound7). These segments were further grouped into four zones (G1–G4) from the leaf apex to the base to facilitate visualization and analysis. b Each leaf segment was imaged under a microscope at 10X magnification to visualize the variation of chloroplast clusters in the interveinal regions. The lamina between venules within each segment was then imaged at 20X magnification to visualize stomatal variation and morphology. Outlines of guard cells are provided for illustrative purposes. Representative images of chloroplast clusters for G1 and G4 zones are also shown at 10X magnification to illustrate differences in size and to provide examples of the clusters analyzed in this study (scale bar = 100 μm).
