Fig. 4: Recognition of Bbss by selected aptamers using flow cytometry. | Communications Biology

Fig. 4: Recognition of Bbss by selected aptamers using flow cytometry.

From: Selection and characterization of DNA aptamers targeting the surface Borrelia protein CspZ with high-throughput cross-over SELEX

Fig. 4

Incubation with 500 nM of AptaCtrl in black a Aptamers considered as not interacting with the bacteria Bbss, mirroring the AptaCtrl. b Aptamers considered as interacting with Bbss in comparison with AptaCtrl. The black line highlights the negative control signal c Recognition of Bbss by selected aptamers (mean fluorescence intensity evaluated using flow cytometry). Incubation without aptamers (in white), or with 500 nM of AptaCtrl (black), Apta7 (grey), Apta9 (orange), or Apta10 (blue). Individual data points are represented by circular, triangular, square and rhombic markers. The error bars represent the standard deviation associated with the mean fluorescence intensity from n=3 biologically independent experiments. Two-sample t-test was applied for the statistical analysis (*p<0.05, and ***p<0.001). d Sequence alignment of Apta9 and Apta10, generated using Multalin (v5.4.1). Identical nucleotides between the two aptamers are highlighted in red.

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