Table 1 Quantitative analysis of the interaction between immobilized aptamers and CspZ by BLI
Sequence | KD (nM) | kon (M−1.s−1) | koff (s−1) | χ2 | R2 |
|---|---|---|---|---|---|
AptaCtrl | No interaction observed at 10 µM CspZ | ||||
1 | 565.4 ± 2.0 | 29.4 | 166.5 × 10−7 | 117.4 | 0.999 |
2 | 534.5 ± 1.5 | 36.1 | 192.9 × 10−7 | 169.3 | 0.999 |
3 | 47.4 ± 1.2 | 191.7 | 90.9 × 10−7 | 14.9 | 0.997 |
4 | 37.8 ± 1.0 | 178.2 | 67.4 × 10−7 | 16.4 | 0.998 |
5 | 1015.0 ± 1.6 | 34.4 | 349.1 × 10−7 | 158.0 | 0.999 |
6 | 54.0 ± 0.3 | 153.0 | 82.7 × 10−7 | 12.3 | 0.999 |
7 | 17.4 ± 0.3 | 246.1 | 42.8 × 10−7 | 79.6 | 0.997 |
8 | 701.9 ± 1.9 | 33.7 | 236.2 × 10−7 | 148.7 | 0.999 |
9 | 105.1 ± 0.7 | 244.4 | 256.8 × 10−7 | 44.5 | 0.998 |
10 | 90.6 ± 0.8 | 324.3 | 293.7 × 10−7 | 127.8 | 0.997 |
11 | 10.6 ± 0.1 | 843.8 | 89.3 × 10−7 | 120.6 | 0.994 |
12 | 187.7 ± 0.6 | 157.1 | 294.8 × 10−7 | 29.8 | 0.998 |
13 | 975.4 ± 1.5 | 33.8 | 329.5 × 10−7 | 116.2 | 0.998 |
