Fig. 2: SscLLs expression in different tissues of S. schlegelii and subcellular localization in HEK293T cells. | Communications Biology

Fig. 2: SscLLs expression in different tissues of S. schlegelii and subcellular localization in HEK293T cells.

From: The role of ladderlectin in spermatogenesis and ovarian sperm storage in the black rockfish (Sebastes schlegelii)

Fig. 2

a Heatmap of the expression of SscLLs. The horizontal axis represents each tissue of S. schlegelii, the vertical axis represents the gene IDs, and the scale bar indicates the normalized TPM value. b PCR products of SscLL3604 and SscLL3605 gene fragments in different tissues of S. schlegelii. H heart, L liver, S spleen, K kidney, B brain, G gill, M muscle, I intestine, O ovary, T testis. c Subcellular localization of pEGFP-N1-SscLL3604 and pDsRed2-N1-SscLL3605. The nucleus of HEK293T cells were stained with DAPI, the green fluorescence represented pEGFP-N1-SscLL3604 or pEGFP-N1, and the red fluorescence represented pDsRed2-N1-SscLL3605 or pDsRed2-N1. Scale bars, 30 μm.

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