Fig. 3: The spatial expression of SscLLs genes mRNA and protein in S. schlegelii testis at different developmental stages during the annual reproductive cycle.

a–p Localization of SscLL3604 and SscLL3605 gene mRNA by ISH. Positive signals from hybridization with antisense probes are shown in blue, and negative signals from hybridization with sense probes are uncolored. q–x The spatial expression of SscLLs protein using IHC. The positive signal of IHC was shown to be yellow-brown, and the negative control tissues were stained blue with hematoxylin. Rs stage Regressed Stage, Rg stage Regenerating Stage, Sg stage_Sep Spermatogenesis Stage in September, Sg stage_Nov Spermatogenesis Stage in November, PSc Primary spermatocytes, SSc Secondary spermatocytes, St Spermatid, Sz Spermatozoa, LC Leydig cell, SC Sertoli cell. Scale bars, 50 μm, 40 μm.