Fig. 6: The effects of SscLLs in the long-term ovarian sperm storage and embryonic development process of S. schlegelii.
a Movement trajectories of sperm within the ovaries of S. schlegelii in control and experimental groups at different time points. Black arrows indicate spermatozoa. PG Oocyte at the 2 phase (primary growth), SGl Oocyte at the 4 phase (late secondary growth), SGf Oocyte at the 5 phase (full secondary growth), Gc granulosa cell, Tc theca cell. Scale bars, 40 μm, 15 μm. b–d Representative embryos photographed under the microscope. Scale bars, 300 μm. e Comparison of fertilization rates between the control group and the experimental group (ncontrol = 7208; ntunicamycin = 6093; ninhibitor = 5286; nantibody = 7320, and the detailed data are listed in Supplementary Table S5. n, the total number of fertilized and unfertilized eggs counted). The ratio of normal embryos (f), defective embryos (g), and undeveloped embryos (h). Vertical bars represent the mean ± SD (ncontrol = 7208; ntunicamycin = 6093; ninhibitor = 5231; nantibody = 7317, and the detailed data are listed in Supplementary Table S6. n, the total number of embryos counted), and the horizontal axis represents different groups. ****P < 0.0001 as assessed using two-tailed Welch’s t test.
