Fig. 1: Experimental procedure.
From: Assessing phototoxic drug properties of hydrochlorothiazide using human skin biopsies
Skin biopsies were collected <24 h post-mortem from the hip of body donors. After sampling, biopsies were pre-incubated with either 1 mmol/L hydrochlorothiazide (HCT) dissolved in dimethyl sulfoxide (DMSO) or vehicle (DMSO, untreated control, Ctrl). 8-Methoxypsoralen (8-MOP, 93,75 µg/ml) was used as positive control. After 12 h, skin biopsies were placed into an UVP-crosslinker and irradiated from above without lid or cell culture medium with (I) either low dose 300 mJ/cm2 of ultraviolet radiation type A (UVA; 365 nm) or B (UVB; 302 nm) or with (II) high dose 5 J/cm2 UVA. Following irradiation, biopsies were incubated with either HCT, vehicle, or 8-MOP accordingly. Unirradiated biopsies served as group-specific control. Six and 24 h after irradiation, skin biopsies were harvested and processed for toxicology, isolation of total RNA and protein, as well as for immunohistology. Figure 1 was drawn using some schematic art images modified from Servier Medical Art, Copyright © 2006, Les Laboratories Servier (https://smart.servier.com).
