Table 1 Altered mRNA expression of marker genes involved in apoptosis and tumorigenesis following UV-irradiation

From: Assessing phototoxic drug properties of hydrochlorothiazide using human skin biopsies

 

Ctrl

Ctrl + UVA

Ctrl + UVB

HCT

HCT + UVA

HCT + UVB

Relative gene expression/GAPDH

6 h

Vitamin D-Receptor (VDR)

1.01 ± 0.001

0.93 ± 0.05

0.60 ± 0.1

P = 0.0006 vs. Ctrl

P = 0.0051 vs. Ctrl+UVA

0.91 ± 0.09

0.91 ± 0.07

0.62 ± 0.1

P = 0.0380 vs. HCT

P = 0.0403 vs. HCT + UVA

P2X7- Receptor (P2RX7)

1.02 ± 0.005

0.96 ± 0.05

0.33 ± 0.1

P = 0.0034 vs. Ctrl

P = 0.0281 vs. Ctrl+UVA

0.86 ± 0.1

1.05 ± 0.1

0.39 ± 0.1

P = 0.0205 vs. HCT

P = 0.0016 vs. HCT + UVA

Bcl-2

1.01 ± 0.01

1.11 ± 0.2

0.98 ± 0.06

1.10 ± 0.2

1.17 ± 0.3

1.14 ± 0.2

Bak1

1.02 ± 0.01

1.25 ± 0.2

0.75 ± 0.1

1.55 ± 0.4

1.74 ± 0.4

1.38 ± 0.4

Bax

1.01 ± 0.006

1.10 ± 0.1

0.97 ± 0.1

1.17 ± 0.2

1.87 ± 0.3

1.35 ± 0.2

Relative gene expression/GAPDH

24 h

Vitamin D-Receptor (VDR)

1.00 ± 0.002

1.05 ± 0.09

0.75 ± 0.06

P = 0.0282 vs. Ctrl

P = 0.0117 vs. Ctrl+UVA

0.95 ± 0.08

0.99 ± 0.06

0.76 ± 0.09

P2X7- Receptor (P2RX7)

1.00 ± 0.001

1.09 ± 0.01

0.51 ± 0.1

P = 0.0043 vs. Ctrl+UVA

1.17 ± 0.2

1.09 ± 0.04

0.57 ± 0.1

P = 0.0040 vs. HCT

P = 0.0109 vs. HCT + UVA

Bcl-2

1.01 ± 0.002

0.86 ± 01

0.87 ± 0.2

0.94 ± 0.04

1.11 ± 0.1

0.87 ± 0.1

Bak1

1.00 ± 0.002

1.20 ± 0.2

1.06 ± 0.2

1.01 ± 0.1

1.52 ± 0.4

1.08 ± 0.2

Bax

1.00 ± 0.002

1.02 ± 0.1

1.26 ± 0.2

1.10 ± 0.1

1.32 ± 0.1

1.49 ± 0.2

  1. Data are shown as mean ± SEM. For vitamin D-Receptor (VDR) and P2X7-Receptor (P2RX7) n = 6 biopsies per group, for Bcl-2, Bak1, and Bax n = 5 biopsies per group. For comparison of three groups P-value was determined using Kruskal-Wallis with Dunn´s multiple comparisons test for Bcl-2, Bak1 and Bax in all groups and in Ctrl group to determine P2RX7 at 6 h and 24 h. One-way ANOVA with Tukey multiple comparisons test was used for VDR of all groups and for the HCT group determine P2RX7at 6 and 24 h. Numerical source data are provided within the Supplementary Data 1 file. Gene names are written in italic.
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