Fig. 3: Inhibition of Wnt signaling is involved in male and female THY1⁺ C1.

A Heat map showing correlations between THY1⁺ clusters of each tissue based on scRNA-seq data. Pearson correlation coefficients are displayed. B Venn diagram showing highly expressed genes of THY1⁺ C1 among AP-m, AP-f, and FP. The 54 genes of THY1⁺ C1 between AP-m and AP-f and the 65 genes of THY1 + C1 among AP-m, AP-f, and FP are shared and highlighted by a red dashed box. Genes that are highly relevant to the context of the study are highlighted in colors. C Dot plot showing DAVID-enriched Gene Ontology terms using 119 (54 + 65) shared highly expressed genes of THY1⁺ C1. D Violin plot showing the expression of WNT inhibitors (SFRP2, SFRP4, DKK2, and DKK3) in THY1⁺ clusters of each tissue and APPCs. Note the significantly high expression levels of these WNT inhibitors in THY1⁺ C1 among AP-m, AP-f, and FP. E Violin plot showing the expression of signature genes of APPCs (RUNX2, TNC, TNN, and PTN) in THY1⁺ clusters of each tissue and APPCs. Note that these signature genes are not expressed in LP.