Fig. 2: Inter-individual variation in gene counts is greater in Xenobots than in age-matched Xenopus embryos for most genes. | Communications Biology

Fig. 2: Inter-individual variation in gene counts is greater in Xenobots than in age-matched Xenopus embryos for most genes.

From: Basal Xenobot transcriptomics reveals changes and novel control modality in cells freed from organismal influence

Fig. 2

A Schematic of the analysis method. All genes were ranked by their mean count value across all 6 data pools, after which the standard deviations of the gene counts amongst individual Xenobots and individual age-matched Xenopus embryos were estimated (Est. σX and Est. σE) and used to calculate the coefficients of variation (CVs) for each (CVX and CVE). B Histograms of the normalized distributions of CVs for Xenobots and age-matched embryos genes. Xenobots have significantly higher CVs and wider CVs distribution than age-matched embryos (Wilcoxon rank sum test, p = 0). Where the CVE distribution had a mean of 1.2537 and standard deviation of 0.9716, and the CVX distribution had a mean of 2.6115 and standard deviation of 1.3609. C Comparison of gene expression variation between Xenobots and age-matched embryos across expression levels. The genes for which CVX > CVE were noted, and of all the included genes, 96.06% had greater CVX than CVE. The gene list was then divided into 100 equal-size bins (percentiles), and the fraction of each bin for which CVX > CVE was plotted. Each bar represents one bin of genes ranked from lowest to highest gene counts, and its height indicates the fraction of genes in the bin for which CVX > CVE. The black line marks 0.5. For all bins, most genes in the bin had a greater CV for Xenobots than age-matched Xenopus embryos. The red line shows the overall fraction of genes for which CVX > CVE (0.9606), and the dark blue bins were found to be significantly different from this value (p < 0.05), while light blue bins were not (p > 0.05) according to a permutation test, indicating that the trend of increasing bin value with increasing gene count was not due to chance. D Table showing top 10 most variable genes in Xenobots.

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