Fig. 2: Spermine block of WT and mutant hTRPV3 channels.
From: Molecular basis of TRPV3 channel blockade by intracellular polyamines
A–F Representative traces of the 2-APB-induced hTRPV3 currents (WT and indicated mutants), measured using a voltage-step protocol (A, top) in absence (black traces, controls) and presence (color-coded traces) of 100 μM cytoplasmic spermine. G Averaged (n = 6 for WT, n = 7 for L639M, n = 5 for D641N, ± SE) Grel-V relationships for WT hTRPV3 (solid red line) and selectivity filter L639M (dashed violet line) and D641N (dashed dark yellow line) mutants in the presence of 100 μM cytoplasmic spermine. H Averaged (n = 6 for WT, n = 5 for all mutants, ± SE) Grel-V relationships for WT hTRPV3 (red line), single E679Q mutant (blue line), single E682Q mutant (green line), double E679Q/E682Q mutant (orange line), and triple E679Q/E682Q/D641N mutant (dashed dark yellow line) in presence of 100 μM cytoplasmic spermine. Datasets were fitted with the sum of two Boltzmann distributions except E679Q/E682Q/D641N mutant, which was fitted with a linear regression. WT TRPV3 data is taken from ref. 43.
