Fig. 3: Blocking of hTRPV3-K169A by polyamines.
From: Molecular basis of TRPV3 channel blockade by intracellular polyamines
A Averaged (n = 6 for WT, n = 7 for K169A, n = 5 for E679Q/E682Q, n = 5 for K169A/E679Q/E682Q, ± SE) Grel-V relationships for WT TRPV3 (solid red line), TRPV3-K169A (dashed red line), TRPV3-E679Q/E682Q (solid orange line), and TRPV3-K169A/E679Q/E682Q (dashed orange line) in presence of 100 μM intracellular spermine. B Averaged (n = 5 for all, ± SE) Grel-V relationships for WT TRPV3 (solid pink line), normalized WT TRPV3 (dashed pink line), and TRPV3-K169A (solid yellow line), in the presence of 100 μM intracellular NASPM, and TRPV3-K169A in the presence of 10 μM (teal line) and 1 μM (brown line) intracellular NASPM. Similarity of K169A and normalized WT Grel-V relationships suggests voltage-independent inactivation mode of WT TRPV3 in the presence of NASPM, which is absent for K169A mutant. Datasets were fitted with Boltzmann distributions (Function 1). Unscaled WT TRPV3 data is taken from ref. 43. C Averaged (n = 5 for all, ± SE) Grel-V relationships for TRPV3-K169A (solid red line), TRPV3-K169A-E679Q (solid blue line), TRPV3-K169A-E682Q, and TRPV3-K169A (solid orange line), in the presence of 100 μM intracellular NASPM, as well as TRPV3-K169A in presence of 10 μM intracellular NASPM (teal line). Datasets were fitted with Boltzmann distributions (Function 1), fitting results are presented in Table S2. (D) Molecular structures of spermine and NASPM, respectively.
