Table 3 Optimized parameters used in Fig. 6C, D

From: Molecular basis of TRPV3 channel blockade by intracellular polyamines

 

Spermine (100 µM)

NASPM (100 µM)

Parameter

WT

E679Q

E682Q

E67Q-E682Q

K169A

K169A-E679Q

K169A-E682Q

K169A-E679Q-E682Q

Ka1

3

2

0.5

0.2

0.3

0.1

0.09

0.06

Z1*

1

1

1

1

1

1

1

1

Ka-1

25

50

60

70

4.00

4.75

5.75

6.00

Z-1*

−1

−1

−1

−1

−1

−1

−1

−1

Ka2

35

80

110

400

0.055

0.075

0.4

11

Z2*

1.6

1.6

1.6

1.6

1.2

1.2

1.2

1.2

KC*

6

6

6

6

2

2

2

2

ZC*

0

0

0

0

0

0

0

0

KP = Ka2/ Ka-1

1.4

1.6

1.8

5.7

1.4*10−2

1.6*10−2

7*10−2

1.8

  1. Parameters labeled with an asterisk (*) were fixed.
  2. Transitions to and from the closed state were considered voltage-independent (i.e. net charge ZC ≡ 0).
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