Fig. 4: CircFNDC3B disrupts TFII-I/STAT1 complex formation. | Communications Biology

Fig. 4: CircFNDC3B disrupts TFII-I/STAT1 complex formation.

From: Tumor cell-intrinsic circular RNA circFNDC3B attenuates CD8+ T cells infiltration in non-small cell lung cancer

Fig. 4

A Co-IP experiments using anti-TFII-I or IgG antibody detected the interaction between TFII-I and STAT1 in A549 cells. B Western blot assay using the indicated antibodies in A549 or H226 cells stably transfected with vector control or TFII-I overexpression plasmid. The enrichment of CXCL10 (C) and CXCL11 (D) promoter fragments with anti-STAT1 or IgG antibody by ChIP-qPCR in A549 cells. Data were displayed with mean ± SD. (Student’s t-test, n = 3, ***P < 0.001). E CXCL10 or CXCL11 promoter luciferase activities were determined after knockdown of STAT1 or overexpression of TFII-I in A549 cells. Data were displayed as mean ± SD. (Student’s t-test, n = 3, **P < 0.01, ****P < 0.0001). F Co-IP experiments about the interaction between TFII-I and STAT1 using anti-TFII-I antibody after overexpression of circFNDC3B or TFII-I in A549 cells. G Western blot assay using the indicated antibodies in A549 cells stably transfected with or without circFNDC3B and TFII-I overexpression plasmids. H CXCL10 or CXCL11 promoter luciferase activities were analyzed in A549 cells stably transfected with or without circFNDC3B and TFII-I overexpression plasmids. Data were displayed as mean ± SD. (Student’s t-test, n = 3, **P < 0.01, ***P < 0.001). I CXCL10 or CXCL11 promoter luciferase activities were analyzed in A549 cells which were stably transfected with or without circFNDC3B and STAT1 overexpression plasmids. Data were displayed as mean ± SD. (Student’s t-test, n = 3, **P < 0.01, ***P < 0.001).

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