Fig. 6: Ancestral sequence reconstruction (ASR) and phylogenetic trees of plant GH3 members. | Communications Biology

Fig. 6: Ancestral sequence reconstruction (ASR) and phylogenetic trees of plant GH3 members.

From: The structure and dynamics of water molecule networks underlie catalytic efficiency in a glycoside exo-hydrolase

Fig. 6

Rooted tree of representative plant GH3 members (117 entries; annotated by NCBI accessions) with putative β-d-glucan glucohydrolase (EC 3.2.1.-) activity in 12 monocot and dicot entries (pink circles and oval) reveals ancestral nodes 179, 180, 195 and 196 (red circles). Entries in blue and blue nodes indicate bacterial sequences bordering plant sequences. The tree generated through FireProtASR26,27 and visualised in FigTree v1.4.381 is based on the HvExoI sequence at 20–90% sequence identity to other entries as input. All plant sequences carry conserved catalytic D285 and E491, and N219, E220 and K260 residues involved in catalysis and water coordination, respectively. The percentage of replicate trees with taxa clustering is shown at node bipartitions. Substitutions per site scale are indicated.

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