Table 1 Relative rates of hydrolysis of poly- and oligosaccharides, and an aryl-glycoside of recombinant HvExoI wild-type (WT) and mutant E220A forms

From: The structure and dynamics of water molecule networks underlie catalytic efficiency in a glycoside exo-hydrolase

Substrate

Activity (μmole/min per mg protein)a

WTb

E220A

(1,3;1,6)-β-d-Glucan (laminarin; 3:2)c

(1,4;1,3)-β-d-Glucan (barley glucan; 2.3-2.7:1)c

54.4 ± 0.5

5.4 ± 0.3

0.4 ± 0.01

ndd

Sophorose

6.0 ± 0.1

1.5 ± 0.10

Laminaribiose

1.3 ± 0.01

1.6 ± 0.06

Cellobiose

4.6 ± 0.2

0.3 ± 0.03

Gentiobiose

4.7 ± 0.4

0.6 ± 0.02

4NP-Glc

12.4 ± 0.8

1.6 ± 0.08

  1. aData, expressed in one or two significant digits, are given as mean values with standard error of measurements (n = 2) after ± signs.
  2. bFrom ref. 22.
  3. cMain types linkages and the ratio of linkage type84.
  4. dnd, (activity) not detected.
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