Table 3 Apparent inhibition constants of recombinant HvExoI wild-type (WT) and mutant E220A forms
Kia | |||
|---|---|---|---|
Inhibitor | WT glycosylated | WT De-glycosylated | E220A De-glycosylated |
G6SG-OMe Ki (M) ΔG (kJ mol−1)b | 109 × 10−6 − 23.0 | 71 × 10−6 − 24.1 | 340 × 10−6 − 20.1 |
Glucono-δ-lactone Ki (M) ΔG (kJ mol−1) | 10 × 10−6 − 29.0 | 8 × 10−6 − 29.6 | 103 × 10−6 − 23.1 |
2F-DNPGlc Ki (M) ΔG (kJ mol−1) | 0.5 × 10−6 − 36.6 | 0.5 × 10−6 − 36.6 | 0.5 × 10−6 − 36.6 |
